Journal: Gut Microbes

Article Title: Responses of intestinal organoids to infection by Mycobacterium avium resemble symptoms observed in Crohn’s disease

doi: 10.1080/19490976.2026.2630483

Figure Lengend Snippet: MMP7 is upregulated in M. avium -infected mSIOs and in the proximal colon of humanized CD mice. (A) qRT-PCR validation of Mmp7 expression in M. avium -infected mSIOs and their uninfected control mSIOs. (B) Western blot shows the higher MMP7 protein level in M. avium- infected mSIOs compared to the control. (C) MMP7 immunostaining in the M. avium- infected organoids and the uninfected control. Bottom panel shows higher magnification images (63 × , N.A. 1.4) of M. avium -infected organoids in the top panel (20 × , N.A. 0.75).

Article Snippet: Membranes were blocked with 5% non-fat milk in TBS-T (Tris-buffered saline with 0.1% Tween-20) for 1 hour at room temperature and incubated overnight at 4 °C with primary antibodies MMP7 (3801, Cell Signaling D4H5) and β -actin (4970, Cell Signaling 13E5) (loading control).

Techniques: Infection, Quantitative RT-PCR, Biomarker Discovery, Expressing, Control, Western Blot, Immunostaining

Journal: Nature Communications

Article Title: Injured epithelial cell states impact kidney allograft survival after T-cell-mediated rejection

doi: 10.1038/s41467-026-68397-1

Figure Lengend Snippet: A SnRNA-seq UMAP plot (left) of PT subclustering including anatomical segments (PT S1-3, PT S3 med.), a proliferative PT cluster (PT Prolif) and injured clusters (PT Injury m1-4). Pseudotime analysis using partition-based graph abstraction (PAGA) highlighting diffusion pseudotime (right). B Spatial localization of PT injury clusters derived from snRNA-seq label transfer to ST data. C Heatmap with expression of marker genes across PT subclusters, maximum-normalized on a per-gene basis. Genes present in the Xenium panel are marked with an asterisk (compare to Suppl. Fig. ). D Relative abundances of PT subclusters across different transplant groups. E Immunofluorescence staining of PT Injury m4 marker genes VCAM1, ANXA3, KIM1 and canonical PT marker LRP2 in a C57BL/6 to BALB/c kidney (see Suppl. Fig. for remaining groups). Consistent with snRNA-seq predictions, VCAM1, KIM1 and ANXA3 share overlapping expression domains that diminish in size from VCAM1 to KIM1 to ANXA3. Nuclei are counterstained with DAPI. Experiment repeated three times with similar result. F – I Analogous plots for the TAL. J Immunofluorescence staining validates the TAL Injury m3 marker MMP7, detected in scattered medullary NKCC2-positive TAL cells in allogeneic but not syngeneic grafts. Nuclei are counterstained with DAPI. MMP7 also labels PT cells. Remaining groups are shown in Supplementary Fig. . Experiment repeated three times with similar result. D , I show results from two-sided Student’s t-test with Benjamini-Hochberg correction for multiple testing. Adjusted P -value: *<0.05, **<0.005 (for D , I ). Scale bar ( B , G ): 500 µm, scale bar large images ( E , J ): 1000 µm, scale bar small images ( E , J ): 50 µm. Source data are provided as a Source Data file.

Article Snippet: Rabbit Polyclonal MMP7 Antibody 0,6 mg/ml , Proteintech , 1:200 , Cat. # 10374-2-AP RRID: AB_2144452 LOT: 00101692.

Techniques: Diffusion-based Assay, Derivative Assay, Expressing, Marker, Immunofluorescence, Staining

Journal: Nature Communications

Article Title: Injured epithelial cell states impact kidney allograft survival after T-cell-mediated rejection

doi: 10.1038/s41467-026-68397-1

Figure Lengend Snippet: A Representative overview and inset images of proximal tubule injury showing STK39 (red), ANXA3 (magenta), KIM1 (cyan), ACSM3 (yellow), VCAM1 (gray), LRP2 (green) and DAPI (blue). Arrowheads mark proximal tubules expressing different injury marker combinations. B Representative overview and inset images of TAL injury showing NKCC2 (green, encoded by SLC12A1 ), MMP7 (yellow) and DAPI (blue). Arrowheads mark TAL cells expressing MMP7 (TAL Injury h2) in medullary portions of the biopsy. C Representative overview and inset images of inflammatory microenvironments around PT Injury h2 cells comprising T-cells (CD8A, magenta), macrophages (CD68, orange) and fibroblasts (ACTA2, green). Injured PT cells are identified by expression of LRP2 (red) and KIM1 (cyan). Nuclei are stained with DAPI (blue). Asterisks mark uninjured proximal tubules. A – C Scale bars of overview images: 1000 µm. Scale bars of insets: 50 µm. All experiments repeated three times with similar result.

Article Snippet: Rabbit Polyclonal MMP7 Antibody 0,6 mg/ml , Proteintech , 1:200 , Cat. # 10374-2-AP RRID: AB_2144452 LOT: 00101692.

Techniques: Expressing, Marker, Staining

Journal: F1000Research

Article Title: A guide to selecting high-performing antibodies for MMP7 (UniProt ID: P09237 ) for use in western blot and immunoprecipitation

doi: 10.12688/f1000research.175973.1

Figure Lengend Snippet: Culture media from A549 WT and MMP7 KO cells were collected, and 30 μg of protein was processed for western blot with the indicated MMP7 antibodies. The Ponceau stained transfers of each blot are presented to show equal loading of WT and KO samples. Antibody dilutions were chosen according to the recommendations of the antibody supplier. Antibody dilutions used: ab176325** at 1/1000, ab205525** at 1/1000, ab207299** at 1/1000, 71031 at 1/1000, GTX104658 at 1/1000, NB110-60988* at 1/1000, 10374-2-AP at 1/1000, 67990-1-Ig* at 1/2000, MAB9071* at 1/500 and MA5-51356** at 1/1000. Predicted band size: 29.7 kDa. *Monoclonal antibody; **Recombinant antibody.

Article Snippet: Proteintech , 10374-2-AP , 00079668 , AB_2144452 , Polyclonal , - , Rabbit , 600 , ICC/IF, IHC, WB.

Techniques: Western Blot, Staining, Recombinant

Journal: F1000Research

Article Title: A guide to selecting high-performing antibodies for MMP7 (UniProt ID: P09237 ) for use in western blot and immunoprecipitation

doi: 10.12688/f1000research.175973.1

Figure Lengend Snippet: Protein lysates from A549 WT and MMP7 KO cells were collected, and 30μg of protein was used for western blot with the indicated MMP7 antibodies. The Ponceau stained transfers of each blot are presented to show equal loading of WT and KO samples. Antibody dilutions were chosen according to the recommendations of the antibody supplier. Antibody dilutions used: ab176325** at 1/1000, ab205525** at 1/1000, ab207299** at 1/1000, 71031 at 1/1000, GTX104658 at 1/1000, NB110-60988* at 1/1000, 10374-2-AP at 1/1000, 67990-1-Ig* at 1/2000, MAB9071* at 1/500 and MA5-51356** at 1/1000. Predicted band size: 29.7 kDa. *Monoclonal antibody; **Recombinant antibody.

Article Snippet: Proteintech , 10374-2-AP , 00079668 , AB_2144452 , Polyclonal , - , Rabbit , 600 , ICC/IF, IHC, WB.

Techniques: Western Blot, Staining, Recombinant